CD38 produces nicotinic acid adenosine dinucleotide phosphate in the lysosome

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Nicotinic Acid Adenine Dinucleotide Phosphate (NAADP

Microinjection of human Jurkat T-lymphocytes with nicotinic acid adenine dinucleotide phosphate (NAADP 1 ) dose-dependently stimulated intracellular Ca 2 1 -signaling. At a concentration of 10 nM NAADP 1 evoked repetitive and long-lasting Ca 2 1 oscillations of low amplitude, whereas at 50 and 100 nM, a rapid and high initial Ca 2 1 -peak followed by trains of smaller Ca 2 1 -oscillations was o...

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Nicotinic acid adenine dinucleotide phosphate (NAADP+).

Microinjection of human Jurkat T-lymphocytes with nicotinic acid adenine dinucleotide phosphate (NAADP 1 ) dose-dependently stimulated intracellular Ca 2 1 -signaling. At a concentration of 10 nM NAADP 1 evoked repetitive and long-lasting Ca 2 1 oscillations of low amplitude, whereas at 50 and 100 nM, a rapid and high initial Ca 2 1 -peak followed by trains of smaller Ca 2 1 -oscillations was o...

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CD38 is the major enzyme responsible for synthesis of nicotinic acid-adenine dinucleotide phosphate in mammalian tissues.

In the present study, we have determined the role of the enzyme CD38 upon the synthesis of the Ca(2+)-releasing nucleotide nicotinic acid-adenine dinucleotide phosphate (NAADP). In rat tissues, we observed that the capacity for NAADP synthesis could be co-immunoprecipitated with CD38 using an anti-CD38 antibody. Furthermore, we observed that several tissues from CD38 knockout mice had no capaci...

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Determination of cellular nicotinic acid-adenine dinucleotide phosphate (NAADP) levels.

Nicotinic acid-adenine dinucleotide phosphate (NAADP) is fast emerging as a new intracellular Ca2+-mobilizing messenger. In sea urchin egg homogenates, binding of NAADP to its receptor is not readily reversible; hence, prior incubation with low concentrations of NAADP is more effective in inhibiting subsequent binding of radiolabelled NAADP than incubating the preparation with the two ligands s...

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Nicotinic Acid Adenine Dinucleotide Phosphate (NAADP)-mediated Calcium Signaling*

Nicotinic acid adenine dinucleotide phosphate (NAADP)2 is a metabolite of NADP that was first identified as the most potent Ca2 stores mobilizing molecule in sea urchin egg homogenates more than a decade ago (1, 2). It has since been shown to be effective in a wide variety of cells, from plant to animal, including human (reviewed in Refs. 3–5). Its mechanism of action is distinct from those of ...

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ژورنال

عنوان ژورنال: Journal of Biological Chemistry

سال: 2018

ISSN: 0021-9258

DOI: 10.1074/jbc.ra118.002113